Products
PRIMACYT Cell Culture Technology GmbH is an ISO certified company on the basis of EN ISO 9001 (2000) and EN ISO 13485 (2003)
PDF-Download: Certificate Quality Management System
Beagle Hepatocytes
Primary Beagle hepatocytes for in vitro studies. The hepatocytes can be cultured in Human Hepatocyte Maintenance Medium for several weeks while hepatocellular functions are maintained.
PDF-Download: Product description Beagle hepatocytes
HGF Hepatocyte Growth Factor
HGF is a growth factor acting as mitogen, motogen, and morphogen for hepatocytes ("3M action") and anti-proliferative on hepatoma cells.
PDF-Download: Product Description
HHMM Human Hepatocyte Maintenance Medium
Serum-free medium for long-term cultivation of human hepatocytes.
PDF-Download: Product description HHMM
HEPAC² Serum-free Hepatocyte Cultures
We offer cell culture systems using chemically defined media. We focus on a high degree of differentiation and a corresponding long-term maintenance of hepatocellular function.
PDF-Download: Product description HEPAC²
Microsomes
Human and animal microsomes for in vitro analysis of cytochrome P450 activities.
PDF-Download: Product description Microsomes
Collagen
Rat tail collagen for coating of cell culture plates.
Quality control
For quality control purposes we measure synthesis and excretion of urea and serum proteins. The viability of our cultures is determined by the release of lactate dehydrogenase (LDH) into the culture medium.
We evaluate the quality of our cells on a daily basis by light microscopy, analysis of hepatocellular functions and LDH-release.
Analysis of the clinical chemical parameters is performed at the Institute of Laboratory Medicine at HELIOS Kliniken, Schwerin (ISO9001:2000 certified). Thereby, we achieve an independent external quality control of our hepatocyte cultures.
Services
1. Standardized and validated serum-free human and rat hepatocyte cultures. In addition, on request
3. Metabolism of drugs / biotransformation: Induction of various cytochrome P450 proteins and monitoring changes in expression of these proteins
Partner
HELIOS-Kliniken Schwerin
PRIMACYT Cell Culture Technology GmbH is an ISO certified company on the basis of EN ISO 9001 (2000) and EN ISO 13485 (2003)
PDF-Download: Certificate Quality Management System
Beagle Hepatocytes
Primary Beagle hepatocytes for in vitro studies. The hepatocytes can be cultured in Human Hepatocyte Maintenance Medium for several weeks while hepatocellular functions are maintained.
PDF-Download: Product description Beagle hepatocytes
HGF Hepatocyte Growth Factor
HGF is a growth factor acting as mitogen, motogen, and morphogen for hepatocytes ("3M action") and anti-proliferative on hepatoma cells.
PDF-Download: Product Description
HHMM Human Hepatocyte Maintenance Medium
Serum-free medium for long-term cultivation of human hepatocytes.
PDF-Download: Product description HHMM
HEPAC² Serum-free Hepatocyte Cultures
We offer cell culture systems using chemically defined media. We focus on a high degree of differentiation and a corresponding long-term maintenance of hepatocellular function.
PDF-Download: Product description HEPAC²
Microsomes
Human and animal microsomes for in vitro analysis of cytochrome P450 activities.
PDF-Download: Product description Microsomes
Collagen
Rat tail collagen for coating of cell culture plates.
Quality control
For quality control purposes we measure synthesis and excretion of urea and serum proteins. The viability of our cultures is determined by the release of lactate dehydrogenase (LDH) into the culture medium.
We evaluate the quality of our cells on a daily basis by light microscopy, analysis of hepatocellular functions and LDH-release.
Analysis of the clinical chemical parameters is performed at the Institute of Laboratory Medicine at HELIOS Kliniken, Schwerin (ISO9001:2000 certified). Thereby, we achieve an independent external quality control of our hepatocyte cultures.
Services
1. Standardized and validated serum-free human and rat hepatocyte cultures. In addition, on request
- Serum-free mouse hepatocyte cultures
- Serum-free HepG2 hepatoma cell cultures
- Culture of additional cell lines
3. Metabolism of drugs / biotransformation: Induction of various cytochrome P450 proteins and monitoring changes in expression of these proteins
- Analysis of protein expression (Western blots)
- HPLC-based analyses of enzymatic activities
- Two-dimensional protein electrophoresis and mass spectrometry
- RNA expression arrays
Partner
HELIOS-Kliniken Schwerin
- Institut für Laboratoriumsmedizin, Prof. Dr. H. G. Lestin
- Klinik für Chirurgie Abt. für Allgemein und Visceralchirurgie, Dr. med. Klaus Dommisch
- Institut für Pathologie, Dr. med. Raoul Hinze
- Department of Pathology, George K. Michalopoulos, MD, PhD
- Department of Pathology, Stephen C. Strom, PhD
- Center for Biological Imaging, Donna Beer Stolz, PhD
- Zentrum für Toxikologie, Prof. Dr. Jan Hengstler
- Institut für Immunologie, Dr. Dirk Koczan
- Proteom Zentrum Rostock, Prof. Dr. Michael O. Glocker
Growth factors improve hepatocyte-specific function
Electron micrograph of human hepatocytes